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Common blood disorders include anemia, bleeding disorders such as hemophilia, blood clots, and blood cancers such as leukemia, lymphoma, and myeloma.

Suggested primary analysis

Purpose

Tail-vein clotting test in warm saline indirectly examines the volume of blood loss before spontaneous staunching. Proposed first-line analysis for potential clotting disorders with second-line analysis by ex vivo coagulation testing.

Purpose

The measurement of various blood metabolites, ions, and enzymes provides a primary screen for  function of major metabolic organs such as kidney, liver, gastrointestinal tract, as well as for lipid and glucose homeostasis. 

A panel of parameters is proposed, including:

  • Electrolytes and ions: Sodium (Na), Potassium (K), Chloride (Cl), Calcium (Ca), Magnesium (Mg), Phosphate (PO4), iron (Fe), and bicarbonate (CO2).
  • Enzymatic activities: Alpha-amylase, aspartate amino transaminase (AST), alanine amino transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), lipase, creatine kinase (CK).
  • Metabolites: glucose, urea, creatinine, uric acid, total proteins, albumin, total bilirubin, triglycerides, free fatty acids, glycerol, total, HDL and LDL cholesterol, fructosamine, ketone bodies (B-HBA), transferrin, ferritin.

Biochem_Clinical Chemistry graph.jpg

Selected plasma chemistry data from female mice (N=20 per line) of common laboratory strains at different ages (Champy et al. Mammalian Genome 2008).  Error bars reflect ± SEM.

Equipment

These tests are performed with an AU-480 automated laboratory work station (Beckman Coulter France SAS, Villepinte, France).

Strain background references

Champy MF, Selloum M, Zeitler V, Caradec C, Jung B, Rousseau S, Pouilly L, Sorg T, Auwerx J. Genetic background determines metabolic phenotypes in the mouse. Mamm Genome. 2008 May;19(5):318-31. Epub 2008 Apr 5.

Purpose

Determination of prothrombine time, activated partial thromboplastin time and fibrinogen activity.

Biochem_Coagulation Platelets graph

Biochem_Coagulation Leukocytes graph

Biochem_Coagulation Erythrocytes graph

Equipment

ST4 semi automated coagulometer (Diagnostiqua Stago, Gennevilliers, France).

Biochem_Coagulation equipement

Recommendations

It is recommended to collect blood by intra-cardiac puncture.

Suggested secondary analysis

Purpose

First-line hematological/immunological analysis of blood or tissue infiltrates for different leukocyte lineages. May be targeted to examine particular immune lineage subclasses.

Exemples:

Thymus: 

  • T cells of ab lineage: CD4/CD8/TCRab 
  • T cells of gd lineage: CD4/CD8/TCRgd 
  • NKT cells: NK1.1/CD3 

Spleen: 

  • B cells: CD19/IgM/IgD/CD80 
  • B cells: CD19/IgM/CD2/CD23 
  • NK cells and NKT cells: DX5/NK1.1/CD3 
  • Macrophages and neutrophils: F4-80/Gr-1/Mac-1 
  • T cells: CD4/CD8/CD3/CD44

Blood:

  • B cell: CD19/mature B cells IgD
  • Granulocyte
  • Monocyte
  • NK cell 
  • T cell : CD3/CD4/CD8
  • Treg cell : CD25

Please see our Immunology dedicated page for more detailed tests.

Equipment

FACS analysis are performed on a LSR II (BD diagnostics, Le pont de Claix, France).

See our Gene expression analysis dedicated page to explore our different related tests.

See our In vivo Viral Transduction dedicated page to explore our frequently used vectors and delivery modes including stereotactic admininistration (intraventricular and cerebellar)

 

Food challenge

Purpose

With several months notice, we can procure and provide a variety of different restriction diets for nutritional analyses in conjuction with our other analyses.

Models and Challenges

Purpose

With several months notice, we can procure and provide a variety of different restriction diets for nutritional analyses in conjuction with our other analyses.

See our In vivo Viral Transduction dedicated page to explore our frequently used vectors and delivery modes including stereotactic admininistration (intraventricular and cerebellar)