Model creation - Personalized genome engineering

Create your customized model !

Our mission is to develop the most adapted, highly physiologically relevant animal models for Your research program. The genetic engineering services are flexible and extensive to help you generate Your custom-made mouse model, from the design of vectors construction to the functional validation of the mouse model. Our deliverables are transgenic or chimera mice, in SPF or SOPF status.

Our services involve:

Homologous Recombination models	CRISPR and Transgenic models
Design of an optimized strategy including prediction of off-target sites	Design of an optimized strategy including prediction of off-target sites
Preparation of all materials for injection (DNA, vectors, ES cells...)	Preparation of all materials for injection (vectors, sgRNA, Cas9 mRNA, ES cells...)
Injection of genetic materials to generate F0 mutated animals	Injection of genetic materials to generate F0 mutated animals
Germline transmitted F1 animals	Genotyping of F0 animals and delivery of F0 candidates
Excision of the selection cassette (optional)	Off-target analysis in F0 founder animals (optional)
	Germline breeding of F0 founders to achieve germ line transmission (optional)
	Off-target analysis in germline transmitted F1 animals (optional)

 

For more detailed information or for guidance from a scientific consultant, please send us a request via email.

Download  PHENOMIN Genetic Engineering booklet (125.5 KB)