Flp and Cre Deleters

To facilitate the use of the new mutant resource developed in the mouse, we have generated Cre and FlpO deleter mice on a pure inbred C57BL/6N background (Birling et al., 2012).

The new transgenic constructs were designed to drive either the Cre or the FlpO recombinases, fused to a specific fluorescent marker (respectively the eGFP or the eYFP) and were inserted in the neutral Rosa26 locus.

They allow a rapid, cost-effective and efficient identification of the carrier individuals through the co-expression of the fluorescent marker. The main advantages of these deleters are:

• Preservation of a pure genetic background when used to remove specific selection cassette or to induce complete loss-of-function allele (pure inbred C57BL/6N background)
• Insertion in the Rosa26 locus
• Simplicity of genotype identification (fluorescence evaluation)
• High and stable recombination efficiency (up to 100%)
• Expression of Cre and FlpO in developing oocytes respectively of the transmission of the Cre and FlpO transgenes

For more information about our Cre systems, please visit our Mouse Cre and CreER^T2 zoo dedicated pages or contact us directly by email.

Publications

• Birling et al., 2012, Highly-efficient, fluorescent, locus directed cre and FlpO deleter mice on a pure C57BL/6N genetic background.

Users publications:

• Bailly J et al, 2020, Targeting Morphine-Responsive Neurons: Generation of a Knock-In Mouse Line Expressing Cre Recombinase from the Mu-Opioid Receptor Gene Locus.
• Johan G Gilet, et al, 2020, Conditional switching of KIF2A mutation provides new insights into cortical malformation pathogeny.
• Meirsman AC et al, 2017, Mice lacking GPR88 show motor deficit, improved spatial learning and low anxiety reversed by delta opioid antagonist.
• Denaës T et al, 2016, The Cannabinoid Receptor 2 Protects Against Alcoholic Liver Disease Via a Macrophage Autophagy-Dependent Pathway.
• Dubost et al, 2015, Conditional depletion of intellectual disability and Parkinsonism candidate gene ATP6AP2 in fly and mouse induces cognitive impairment and neurodegeneration.
• ...

Complete listing on the Mouse CreER^T2 zoo dedicated pages